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Substrate solution is then added and color develops in proportion to the amount of Rat CASP2
The plate has been pre-coated with Human LCN8 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GS
And then biotinylated Canine IL18 Antibody is added and binds to IL18 in the sample
And then biotinylated Human NFASC Antibody is added and binds to NFASC in the sample
Human PAP (Prostatic Acid Phosphatase) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (PAP) Prostatic Acid Phosphatase ELISA Kit measures Prostatic Acid Phosphatase in samples. The plate has been pre coated with Human PAP antibody. PAP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PAP Antibody is added and binds to PAP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PAP antibody. After incubation unbound Streptavidin HRP is washed away
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