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ARHGEF6 present in the sample is added and binds to antibodies coated on the wells
The concentration of Mouse FKBP5 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated P4HA2 antibody
The OD value is proportional to the concentration of Human ISG15
The plate has been pre-coated with Mouse SDC2 antibody
Human PCSK1N (Prosaas) ELISA Kit Packaging:96 wells ARHGEF6 present in the sampleThe Human (PCSK1N) Prosaas ELISA Kit measures Prosaas in samples. The plate has been pre coated with Human PCSK1N antibody. PCSK1N present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PCSK1N Antibody is added and binds to PCSK1N in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PCSK1N antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
